FIG. 1.

(A) Schematic illustration of lentiviral vector expressing both p12^I-HA and GFP (sample vector) as bicistronic messages and GFP alone (control vector) from elongation factor 1α promoter. Abbreviations: LTR, long terminal repeats; RRE, Rev response element; EFIα, elongation factor 1α promoter; IRES, internal ribosome entry site; WPRE, woodchuck hepatitis posttranscriptional regulatory element. (B) Flow cytometric analysis illustrating the expression of GFP in Jurkat T cells 7 days postinfection with lentiviral vectors. Both sample (expressing p12^I-HA and GFP) and control (GFP alone) groups contain relatively high and similar levels of GFP. (C) RT-PCR demonstrating the expression of p12^I-HA in Jurkat T cells 7 days postinfection with lentiviral vectors. Jurkat cells spin infected with sample vector express p12^I whereas control vector spin-infected cells do not express p12^I. RT-PCR was performed with triplicate samples and controls. GAPDH was used as a control for the integrity of the message.