GC-MS chromatograms for A) Melanophryniscus klappenbachi and B) Melanophryniscus cupreuscapularis. Alkaloids were identified from retention times (Rt) and spectral data that are documented in Daly et al., 2005. Major and minor alkaloids are as follows: A: 223F (5), 9.20 min; 223D (6), 9.62 min; 239Q (1), 10.44 min; 249F (3), 10.98 min; 251D (4), 11.11 min; 275I (2), 12.98 min. B: 207S, 7. 63min, (see text); 235S, 9.97 min; 239Q (1), 10.64 min; 249F (3), three isomers, 11.31, 11.83, 12.03 min; 259B, two isomers, 11.37, 11.62 min; 275I (2), 13.20 min. Rts for 1, 2, and 3 differ between A and B, as the analyses were made several years apart. The x’s indicate non-alkaloid impurities.