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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: Virology. 2008 Jan 14;373(2):239–247. doi: 10.1016/j.virol.2007.11.028

Figure 2. Effects of curcumin on HSV-1 IE gene expression.

Figure 2

(A) HeLa cells were pretreated with 20 μM curcumin (+, black bars) or DMSO only (-, open bars) for 3 hours and then infected with HSV-1 at an MOI of 1 pfu/cell. Total RNA was isolated at 2 hpi and gene expression was analyzed by Q-RT-PCR, normalized against 18S rRNA levels. Mean values from three experiments for each gene tested are shown relative to values obtained from untreated cells; error bars represent standard deviations. (B) HeLa cells were infected with HSV-1 in the presence (+, black bars) or absence (-, open bars) of 20 μM curcumin. Aliquots of nuclear extracts (as used in ChIP assays, Fig. 3) were analyzed by Q-PCR. The amount of viral DNA (represented by the ICP27 gene promoter) was normalized against cellular DNA (represented by the U3 snRNA promoter). Error bars represent the standard deviation among five independent experiments.