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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: Virology. 2008 Jan 14;373(2):239–247. doi: 10.1016/j.virol.2007.11.028

Figure 3. Curcumin decreases occupancy of RNA polymerase II but not of VP16 at IE genes.

Figure 3

HeLa cells treated with 20 μM curcumin (+, black bars) or DMSO only (-, open bars) were infected with HSV-1 at an MOI of 5 pfu/cell. Chromatin immunoprecipitation from sonicated nuclear extracts was performed using antibodies against VP16 (A) or RNA pol II (B). Samples were analyzed by Q-PCR using primers specific for the ICP0 promoter, ICP27 promoter, ICP27 ORF or the cellular U3 snRNA promoter. Aliquots of samples prior to immunoprecipitation (1%, 0.3%, 0.1%, 0.04% input) were used to create a standard curve of input DNA. This curve was used to determine the relative amounts of a given DNA fragment in immunoprecipitated or control (no antibody) samples. The values for no-antibody controls were subtracted from the values for corresponding IP samples. Error bars represent the range between technical duplicates for a representative experiment.