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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: Virology. 2008 Jan 14;373(2):239–247. doi: 10.1016/j.virol.2007.11.028

Figure 5. Knocking down p300 expression does not affect HSV-1 IE gene expression.

Figure 5

HeLa cells were transfected with 3 μg of pSUPER (empty) or pSUPER-p300 plasmid that expresses a shRNA targeting p300. 48 hours post-transfection cells were infected with HSV-1 at an MOI of 5 pfu/cell and total protein and RNA were isolated. p300 knockdown efficiency was analyzed by immunoblotting (A) and Q-RT-PCR (B). IE gene expression was analyzed by Q-RT-PCR as indicated in previous figure legends (C). Data represents the average of three independent experiments done at least in biological duplicates. Error bars represent the standard deviation.