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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cell Signal. 2009 Mar 1;21(6):1015–1021. doi: 10.1016/j.cellsig.2009.02.017

Figure 1.

Figure 1

masGRK3ct-cerulean is localized at the plasma membrane, and transfers energy to Gβγ-venus at the plasma membrane. (A) Confocal images of an HEK 293 cell expressing masGRK3ct-C and Gβγ-V; cerulean (C) was excited at 458 nm, and venus (V) was excited at 514 nm. Both proteins are expressed primarily at the plasma membrane. Regions of interest used for measurements in panel B are outlined in solid (for the plasma membrane) and broken (for the cell interior) lines. (B) Activation of A1 adenosine receptors (A1Rs) with adenosine (30 μM) reversibly increased V emission intensity (510–600 nm; yellow lines) and decreased C emission (465–500 nm; blue lines) after excitation at 458 nm, indicating an increase in FRET between masGRK3ct-C and Gβγ-V. The FRET increase occurred largely at that plasma membrane.