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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Cell. 2009 Apr 3;137(1):133–145. doi: 10.1016/j.cell.2009.01.041

Figure 3.

Figure 3

Ub with K48 alone cannot support yeast viability and cumulative K to R substitutions lead to growth defects.

(A) The strategy for switching Ub expression in yeast.

(B) Expression of Ub-K48 as the only Ub source resulted in lethality (1X = ~100 cells).

(C) Growth curves of yeast strains expressing a single Ub gene under the PCUP1 promoter YPD medium.

(D) Comparison of His-myc-Ub monomer and conjugated forms in yeast strains. Total cell lysates (10 μg) were blotted with anti-myc antibodies.

(E) Quantification of polyUb linkages in yeast strains by MS. All values are normalized according to the levels in the wild-type strain and shown as mean and SEM.