Table 2.
Real time PCR analysis of PFE in rdo2
Drosophila Strain |
Biological Samples |
Transcripts (Fold-Change) |
P (ANOVA) | Ct (Gapdh2) | Ct (PFE) |
---|---|---|---|---|---|
w1118 | 3 | 1.0 | 15.57 ± 0.29 | 25.37 ± 1.23 | |
rdo2 | 3 | -1.741 ± 0.37 | 0.1686 | 15.72 ± 0.24 | 27.11 ± 0.86 |
The level of wild-type (w1118) transcript is defined as 1.0. Data (Mean ± SD) are fold-change in level of PFE transcript. ‘-’ indicates a reduction in amount of PFE transcript relative to control (no significant difference). Data are normalized based on difference in average cycle threshold (Ct) for Gapdh2 and PFE.