Table 3.
A List of Glycopeptides That Were Successfully Identified in Deglycosylated Fractions but Went Undetected in Tryptic Digests
| protein name | potential glycosylated site | peptide mass, MH+ | peptide sequence | glycosylation sitea | no. of samples detected |
|---|---|---|---|---|---|
| uromodulin | K.QDFN#ITDISLLEHR.L | 1701.85 | 319–332 | 322 N-linked (GlcNAc…) | 2 |
| prostaglandin-H2 D-isomerase | R.WFSAGLASN#SSWLR.E | 1582.77 | 45–56 | 51 N-linked (GlcNAc…) | 5 |
| K.SVVAPATDGGLN#LTSTFLR.K | 1920.01 | 67–85 | 78 N-linked (GlcNAc…) | 10 | |
| lysosomal α-glucosidase | K.LEN#LSSSEMGYTATLTR.T | 1873.90 | 138–154 | 140 N-linked (GlcNAc…) | 5 |
| R.GVFITN#ETGQPLIGK.V | 1574.85 | 465–479 | 470 N-linked (GlcNAc…) | 5 | |
| R.N#NTIVNELVR.V | 1172.63 | 882–890 | 882 N-linked (GlcNAc…) | 7 | |
| CD44 antigen | K.AFN#STLPTMAQMEK.A | 1569.74 | 55–68 | 57 N-linked (GlcNAc…) | 8 |
| kininogen-1 | K.YNSQN#QSNNQFVLYR.I | 1875.87 | 44–58 | 48 N-linked (GlcNAc…) | 2 |
| K.LNAENN#ATFYFK.I | 1432.68 | 289–300 | 294 N-linked (GlcNAc…) | 7 | |
| α-1-antitrypsin | K.YLGN#ATAIFFLPDEGK.L | 1756.89 | 268–283 | 271 N-linked (GlcNAc…) | 4 |
| clusterin | R.LAN#LTQGEDQYYLR.V | 1725.94 | 372–385 | 374 N-linked (GlcNAc…) | 4 |
| polymeric immunoglobulin receptor | K.VPGN#VTAVLGETLK.V | 1398.79 | 466–479 | 469 N-linked (GlcNAc…) | 6 |
| galectin-3-binding protein | R.ALGFEN#ATQALGR.A | 1348.69 | 64–76 | 69 N-linked (GlcNAc…) | 3 |
| cathepsin D | K.GSLSYLN#VTR.K | 1110.59 | 257–266 | 263 N-linked (GlcNAc…) | 2 |
| lysosomal acid phosphatase | R.YEQLQN#ETR.Q | 1181.55 | 162–170 | 167 N-linked (GlcNAc…) | 3 |
| Ig α-2 chain C region | K.TPLTAN#ITK.S | 959.55 | 200–208 | 205 N-linked (GlcNAc…) | 3 |
| β-glucuronidase | K.VVAN#GTGTQGQLK.V | 1273.69 | 269–281 | 272 N-linked (GlcNAc…) | 4 |
| haptoglobin | K.VVLHPN#YSQVDIGLIK.L | 1796.01 | 236–251 | 241 N-linked (GlcNAc…) | 4 |
| α-1-antichymotrypsin | K.YTGN#ASALFILPDQDK.M | 1753.88 | 268–283 | 271 N-linked (GlcNAc…) | 2 |
| lysosome-associated membrane glycoprotein 2 | K.IAVQFGPGFSWIAN#FTK.A | 1883.98 | 88–104 | 101 N-linked (GlcNAc…) | 3 |
| fibrillin-1 | K.TAIFAFN#ISHVSNK.V | 1549.81 | 2761–2774 | 2767 N-linked (GlcNAc…) | 2 |
| lysosome-associated membrane glycoprotein 1 | K.N#MTFDLPSDATVVLN#R.S | 1794.87 | 61–76 | 61, 75 N-linked (GlcNAc…) | 3 |
| proactivator polypeptide | R.TN#STFVQALVEHVK.E | 1573.83 | 214–227 | 215 N-linked (GlcNAc…) | 2 |
a
Glycosylated sites as annotated in Swiss-Prot database