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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: J Cell Sci. 2004 Oct 12;117(Pt 23):5489–5496. doi: 10.1242/jcs.01398

Fig. 5.

Fig. 5

FRET images of Cdc42 in chemotaxing cells. Membrane-bound Cdc42 activities were tested by FRET assay in HEK 293 cells expressing (A) WT or (B) LLKIL mutant CXCR2. FRET images were made as ratio images from two different fluorescent images and plotted with pseudocolor, in which, red represents the highest activity and blue is lowest. Although the overall activities were increased in both types of cells expressing either receptor, the distribution of Cdc42 activities was only polarized in WT CXCR2-expressing cell with a higher level in the leading edge. The number showing in each image indicates the time-lapse in minutes after ligand application, the arrow indicates the direction of ligand application. Scale bars, 20 μm. Each experiment was repeated at least three times and representative images are shown. The time-lapse video clips are available online (Movies 5 and 6 in supplementary material).