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. Author manuscript; available in PMC: 2009 Apr 13.
Published in final edited form as: J Struct Funct Genomics. 2008 Dec 4;10(1):17–23. doi: 10.1007/s10969-008-9051-x

Fig. 5.

Fig. 5

Chromatography Fractionation of the Detergent-soluble extract of the liver plasma membrane from adenovirus infected mice. Plasma membranes bound to 1nM of [125I] IL-8 were solubilized with 1% dodecylmaltoside, and the detergent soluble extract was adsorbed to Probond (Ni-resin) column and eluted with a linear gradient of Imidazole (0-300 mM) (a); fractions eluted with imidazol and enriched with [125I] IL-8 were adsorbed to a Concanavalin A-agarose column, which was then washed with 1 M NaCl and eluted with a linear gradient of α-methyl glucoside (0-400 mM) (b). Fractions were analyzed for radioactivity and protein concentrations. The high levels of radioactivity in the initial fractions of the columns correspond to free [125I] IL-8, dissociated from the [125I] IL-8-bound to the soluble extract during the chromatography fractionation