TABLE 2.
Reactivity of TcF and ITC6 recombinant proteins in EIA with RIPA-confirmed positive seraa
| Serum panel | No. of sera | TcF EIA
|
ITC6 EIA
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| No. positive | Mean S/CO | SD | 99th percentile | No. positive | Mean S/CO | SD | 99th percentile | ||
| ARC | 42 | 36 | 3.575 | 3.2 | 0.306-11.171 | 42 | 7.545 | 4.877 | 1.387-16.077 |
| BBI | 14 | 13 | 5.485 | 3.084 | 0.368-10.490 | 14 | 10.164 | 3.651 | 4.654-16.402 |
| Controls | 4 | 0 | 0.429 | 0.19 | 0.224-0.644 | 0 | 0.694 | 0.102 | 0.551-0.776 |
a
Data for both antigens are expressed as the mean S/CO and include the 99th percentiles to facilitate comparison of the data. RIPA-confirmed positive sera from the ARC (n = 42) and BBI panel sera confirmed positive by RIPA (n = 14), and normal human serum-negative controls (n = 4) are included. The data indicate improved S/CO and assay sensitivity due to the incorporation of peptides 30, 36, and SAPA.