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Sensitivity and specificity of the one-tube, multiplex, real-time PCR assay for the simultaneous detection, differentiation, and quantification of HTLV-1, -2, and -3. (A) HTLV-1, 10-fold dilutions, 105 to 100 copies per PCR (with standard curve); (B) HTLV-2, 10-fold dilutions, 105 to 100 copies per PCR (with standard curve); (C) HTLV-3, 10-fold dilutions, 105 to 100 copies per PCR (with standard curve); and (D) albumin, 10-fold dilutions, 104 to 100 cells per PCR (with standard curve).
