TABLE 2.
Comparison of CT values of the simplex and multiplex methods
| Beacon probe | Target(s) | 5′ reporter/3′ quencher fora:
|
CT values for 104 copies/PCR
|
||
|---|---|---|---|---|---|
| Single-dye assay | Multiplex assay | Single-dye assay | Multiplex assay | ||
| H1Z1Sd5 | HTLV-1/STLV-1 | FAM/DABCYL | ROX/BHQ1 | 25.1 ± 0.1 | 25.7 ± 0.1 |
| H2Z2Sd3 | HTLV-2 | FAM/DABCYL | FAM/BHQ1 | 25.2 ± 0.3 | 25.1 ± 0.3 |
| H3Z1Sd1 | HTLV-3/STLV-3 | FAM/DABCYL | HEX/BHQ1 | 25.1 ± 0.1 | 25.7 ± 0.2 |
| AlbSd1 | Albumin | FAM/DABCYL | Cy5/BHQ2 | 25.7 ± 0.3 | 25.7 ± 0.5 |
a
FAM, 6-carboxyfluorescein: ROX, 6-carboxy-X-rhodamine; DABCYL, 4-(((4-dimethyl)-phenyl)-azo)benzoic acid.