TABLE 4.
Comparative analysis of results obtained for clinical specimens with the pX primer method and our new multiplex assay
| HTLV status and identity | Sample origin | ELISA result | Western blot pattern | Subtype (by sequencing) | Proviral load (copy no./μg DNA)
|
|
|---|---|---|---|---|---|---|
| pX | Multiplex | |||||
| HTLV-1a | ||||||
| BD DS26* | Blood donor | + | Complete HTLV-1 | NA | 27,602 | 25,277 |
| Gab112LM | Pregnant woman | + | Complete HTLV-1 | A | 22 | 41 |
| Gab683LB | Pregnant woman | + | Complete HTLV-1 | B | 1,200 | 1,924 |
| Gab958LM | Pregnant woman | + | Complete HTLV-1 | B | 36 | 33 |
| Gab70LM | Pregnant woman | + | Complete HTLV-1 | B | 71,100 | 61,061 |
| Gab109LM | Pregnant woman | + | Complete HTLV-1 | B | 76 | 259 |
| Gab826LM | Pregnant woman | + | Complete HTLV-1 | B | 370 | 354 |
| Gab197LM | Pregnant woman | + | Complete HTLV-1 | B | 10 | 6 |
| Gab1014FC | Pregnant woman | + | Complete HTLV-1 | B | 30,800 | 27,454 |
| Gab1058FC | Pregnant woman | + | Complete HTLV-1 | B | 3,450 | 2,920 |
| Gab1144FC | Pregnant woman | + | Complete HTLV-1 | B | 21,260 | 46,123 |
| Gab1123FC | Pregnant woman | + | Complete HTLV-1 | B | 900 | 2,235 |
| Gab1008FC | Pregnant woman | + | Complete HTLV-1 | B | 1,450 | 1,058 |
| Gab1089FC | Pregnant woman | + | Complete HTLV-1 | B | 56,400 | 48,737 |
| HTLV-2a | ||||||
| Gab1080FC | Pregnant woman | + | Complete HTLV-2 | B | Undetectable | 12,259 |
| FE 11355* | Pregnant woman | + | Complete HTLV-2 | NA | Undetectable | 51,800 |
| Indeterminatea | ||||||
| Gab1314PG | Pregnant woman | + | GD21, p19, gp21, p24, p26, p28, p32, p36 | 85 | 108 | |
| Gab1527OY | Pregnant woman | + | p19, gp21, p26, p28, p32, p36 | 0 | 0 | |
| Gab1129FC | Pregnant woman | + | p19, gp21, p24 | 0 | 0 | |
| Gab1004LB | Pregnant woman | + | p19, gp21, p26, p28, p36 | 0 | 0 | |
| STLV-1b | ||||||
| Msp 15G | Wild mandrill | + | Complete HTLV-1 | D | Undetectable | 16,120 |
| Mnd 16B | Wild mandrill | + | Complete HTLV-1 | D | Undetectable | 6,205 |
| Mnd 12 M | Wild mandrill | + | Complete HTLV-1 | D | Undetectable | 9,790 |
| Ms 19 | Wild mandrill | + | Incomplete (no MTA-1) | F | Undetectable | 16,350 |
| Ms 20 | Wild mandrill | + | Complete HTLV-1 | F | Undetectable | 11,556 |
| Ms 25 | Wild mandrill | + | Incomplete (no MTA-1) | F | Undetectable | 3,448 |
| STLV-3 | ||||||
| PPA-F3c | Molecular clone | + | GD21, p24, p36, p53, K55, MTA-1 | NA | Undetectable | 23,504 |
a
The results for the samples obtained from pregnant women and the sequence data were published previously by Etenna et al. (13). An asterisk indicates that data for the sample are newly reported in this paper. The samples were first tested by ELISA and confirmed by Western blotting. NA, not available.
b
The results for the samples obtained from STLV-1-infected monkeys and the corresponding sequences were published by Makuwa et al. (29).
c
The STLV-3 molecular clone obtained from an STLV-3-infected baboon (Papio anubis) was published by Chevalier et al. (8).