TABLE 1.

Reference strains, primer sequences, and multiplex PCR panel assignment for ETEC toxins and colonization factors

Straina	PCR panel	Virulence factor	Product size (bp)	Primer sequence		Reference or source
				Forward (5′-3′)	Reverse (5′-3′)
286 C2	Toxin	LT	273	ACGGCGTTACTATCCTCTC	TGGTCTCGGTCAGATATGTG	11
VX 67356	Toxin	STp	166	TCTTTCCCCTCTTTTAGTCAG	ACAGGCAGGATTACAACAAAG	1
ST 64111	Toxin	STh	120	TTCACCTTTCCCTCAGGATG	CTATTCATGCTTTCAGGACCA	This study
60R936	I	CS1	243	TCCGTTCGGCTAAGTCAGTT	CCGCACATTTCCTGTGTTCT	11
E11881/9	I	CS4	198	ACCTGCGGCAAGTCGTTT	TCTGCAGGTTCAAAAGTCACA	11
E 29101A	I	CS7	154	CGCCGGTTACACGTAGTGAT	CCATTTAAAGTGATTGCGACTT	11
350C1A	I	CS12	137	CCAGTCTATGCCAGGTTGCT	TGTGGGGTCACAGTTTACCA	11
E19446	I	CS3	100	CTAGCTTTGCCACCACCATT	GGCAACTGACTCCCATTTGT	11
E 9034A	II	CS21	617	TCATGAGCCTGCTGGAAGTTATCA	TCCGGCTACCTAAAGTAATTGAGT	4
58R957	II	CS2	368	AGTGGTGGCAGCGAAACTAT	TTCCTCTGTGGGTTCTCAGG	This study
E17018/A	II	CS5	226	TCCGCTCCCGTTACTCAG	GAAAAGCGTTCACACTGTTTATATT	11
258909-3	II	CFA/I	170	GCTTATTCTCCCGCATCAAA	ACTTGTCCTCCCCATGACAC	11
E20738A	II	CS17	130	GGAGACGCTGAATACAACTGA	CTCAGGCGCAGTTCCTTGT	11
E 20738A, CS19	III	CS17/19	195	CGGTGCGTTTAACACAGCTA	TCGATACACTCGCATTCGTT	11
E34420A	III	CS8	166	ATCCGGATTATCAAGCTCCA	GAAGATGTTATTGCACCACCAA	11
E11881/14	III	CS6	152	CTGTGAATCCAGTTTCGGGT	CAGGAACTTCCGGAGTGGTA	11
8786	III	CS15	130	CGAAATTGGACAAGCGATG	TCCAGCAGGGATATTATTCG	11
CS20 Ws 7179A	III	CS20	114	AGGTATCCAAATCCGCACTG	CATCAGCCAGCACATAGGAA	11
CS18	IV	CS18	362	ATCCGTCAGGTGTTTGTGGT	CACCTGAATTCCTCGACAGG	This study
PCFO9	IV	CS13	178	GGGACTGCCACAATGAATTT	CAGCACCACCTGCTGATTTA	11
E7476A	IV	CS14	162	TTTGCAACCGACATCTACCA	CCGGATGTAGTTGCTCCAAT	11
ARG-3	IV	CS22	127	ATTGGACAAGCGTCCAACAC	TTCCAGCAGGGATATTATCATTTT	11

^aAll strains belong to the reference strain collection at Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Göteborg, Sweden.