FIG. 1.

Schematic diagram of the microarray. (A) Illustration of the 50- by 50-mm polymer support with the eight identical, individually addressable array fields. CSNr, chip specification number; ID, identification number. (B) Spotting pattern of one array field. Twenty CCHF virus-specific capture probes were spotted as vertical duplicates in a nine-by-nine pattern with average spot diameters of 325 μm (positions 1 to 20). Four capture probes for the competitive internal control RT-PCR product were included at the bottom of each array (positions 21). Additional functional control probes to visualize successful hybridization and staining were immobilized in three angles of each field (positions C).