TABLE 1.
HIV-1 RNA loads in spiked plasma samples for three dilutions of HIV-1, measured in duplicate by a Biocentric kit after RNA extraction with four RNA extraction kits
| Extraction kit (manufacturer) | Mean plasma log10 copies/ml ± SD (log difference compared to Qiagen kit) at indicated dilution
|
||
|---|---|---|---|
| 3.30 ± 0.40 | 4.31 ± 0.11 | 5.32 ± 0.15 | |
| QIAamp viral RNA mini kit (Qiagen) | 3.38 ± 0.02 | 4.44 ± 0.12 | 5.42 ± 0.02 |
| Sample preparation system (Abbott) | 3.40 ± 0.12 (−0.02) | 4.20 ± 0.25 (0.25) | 5.10 ± 0.15 (0.31) |
| Nuclisens manual extraction kit (bioMérieux) | 3.69 ± 0.06 (−0.31) | 4.34 ± 0.17 (0.11) | 5.42 ± 0.06 (−0.01) |
| High Pure viral nucleic acid kit (Roche) | 2.74 ± 0.24 (0.64) | 4.26 ± 0.34 (0.19) | 5.33 ± 0.19 (0.09) |