. 2009 Feb 4;47(4):1107–1118. doi: 10.1128/JCM.02255-08

TABLE 2.

HIV-1 viral loads in DPS after RNA extraction with four different RNA extraction kits

Spiked plasma dilution^a (log₁₀ copies/ml)	DPS extracted by QIAamp viral RNA mini kit (Qiagen)^b			DPS extracted by Abbott sample preparation system			DPS extracted by Nuclisens manual extraction kit (bioMérieux)^b			DPS extracted by High Pure viral nucleic acid kit (Roche)^b
Spiked plasma dilution^a (log₁₀ copies/ml)	VL	Mean	Plasma − DPS^c	VL	Mean	Plasma − DPS^c	VL	Mean	Plasma − DPS^c	VL	Mean	Plasma − DPS^c
3.30	—			2.85	2.88	0.42	—	3.25	0.05	—
	2.68	NA	0.62	3.15			2.62			—
	—			2.77			3.57			—
	—			2.76			3.56			—
4.31	2.83	2.89	1.42	3.58	3.61	0.70	4.22	4.06	0.24	—
	2.95			3.64			3.91			2.63	NA	1.68
5.32	3.88	3.82	1.50	4.54	4.58	0.74	5.39	5.33	−0.01	3.47	3.28	2.04
	3.98			4.48			4.95			3.71
	3.58			4.67			5.47			2.68
	3.84			4.64			5.51			3.25

DPS were prepared with three different dilutions of human HIV-negative plasma spiked with HIV-1 (Table 1) and were tested in duplicate, and the samples with the lowest and highest viral loads were tested twice in duplicate.

—, undetectable; NA, not applicable.

Difference in log₁₀ copies/ml between the viral loads in plasma and DPS.