TABLE 3.
Amplification of RT and PR regions of the HIV-1 pol gene from DPS prepared with three dilutions of spiked HIV-1 and tested in duplicate after RNA extraction with four RNA extraction kits
| Dilution (log10 copies/ml) | Region amplified | Amplification with RNA extraction kita
|
|||
|---|---|---|---|---|---|
| QIAamp viral RNA mini kit (Qiagen) | Sample preparation system (Abbott) | Nuclisens manual extraction kit (bioMérieux) | High Pure viral nucleic acid kit (Roche) | ||
| 3.30 | RT | − | + | + | − |
| − | + | + | − | ||
| PR | − | − | + | − | |
| − | + | + | − | ||
| 4.31 | RT | − | + | + | − |
| − | + | + | + | ||
| PR | − | + | + | − | |
| − | + | + | + | ||
| 5.32 | RT | + | + | + | + |
| − | + | + | + | ||
| PR | + | + | + | + | |
| − | + | + | + | ||
a
−, no amplification; +, amplification.