TABLE 5.
Amplification of RT and PR regions of the HIV-1 pol gene from each DPS, in duplicate, for three dilutions of spiked HIV-1 after RNA extraction over time and at different ambient temperatures and conditions
| Plasma viral load (log10 copies/ml) | Extraction kit | Amplification of indicated regionb
|
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time 0a
|
1 wk
|
2 wk
|
1 mo
|
2 mo
|
3 mo at 20°C
|
||||||||||||||||
| 20°C
|
37°C
|
20°C
|
37°C
|
20°C
|
37°C
|
20°C
|
37°C
|
||||||||||||||
| RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | RT | PR | ||
| 3.30 | Sample preparation system (Abbott) | + | − | − | + | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Sample preparation system (Abbott) | + | + | − | + | − | − | − | − | − | − | − | + | − | − | − | + | ND | ND | − | − | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | − | + | − | + | − | − | − | − | − | − | − | − | − | − | − | − | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | + | − | + | + | − | + | − | − | − | − | − | − | − | − | − | − | |
| 4.31 | Sample preparation system (Abbott) | + | + | + | + | + | + | − | + | − | + | − | + | − | − | − | + | − | − | − | + |
| Sample preparation system (Abbott) | + | + | + | + | + | + | + | − | − | − | + | + | − | − | − | + | − | − | − | + | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | + | + | + | + | + | + | + | + | − | − | − | + | − | − | − | + | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | + | + | + | + | + | + | − | + | − | − | − | + | − | − | + | + | |
| 5.32 | Sample preparation system (Abbott) | + | + | + | + | + | + | + | + | + | + | + | + | − | − | − | + | − | − | − | − |
| Sample preparation system (Abbott) | + | + | + | + | + | + | + | + | + | + | + | + | − | − | − | + | − | − | − | + | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | − | − | − | + | |
| Nuclisens manual extraction kit (bioMérieux) | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | − | − | − | + | |
a
Results are from Table 3.
b
+, amplification; −, no amplification; ND, not done.