FIG. 5.

CpG composition of ETnII and MusD retrotransposons and promoter activity of in vitro patch-methylated LTRs. (A) CpG composition of ETnII and MusD retrotransposons. The y axis represents overall GC content. CpG dinucleotides, depicted by black vertical bars below, are mapped to a representative ETnII, element 7 (AC079540), and a representative MusD, element 3 (AC084696). Fragments cloned for stable transfection via RMCE (see Fig. 7) are also shown. (B) Relative luciferase activities of methylated and mock-methylated (produced by omitting SssI methyltransferase) LTR-promoted constructs. The data were first standardized to the internal Renilla luciferase control and are expressed relative to the activity of the promoterless cut and religated pGL3B vector. Results are means and standard deviations of three separate experiments performed in duplicate.