FIG. 2.

The majority of c-Jun heterodimerizes with ATF2, and c-Jun/ATF2 heterodimers predominantly occupy the ATF sites in CGNs deprived of potassium. (A) CGNs in 25 K or 5 K medium for 4 h were lysed and subjected to IP with either ATF2 or c-Jun antibodies. The precipitates were analyzed by Western blotting (WB). (B) Cell lysates from CGNs incubated in either 25 K or 5 K medium for 4 h were subjected to immunodepletion (ID) using ATF2 or c-Fos antibodies. The supernatants were analyzed by Western blotting with a c-Jun antibody. The intensity of the c-Jun band was normalized to tubulin. (C) Gel mobility shift assays were performed using ³²P-labeled atf probes or dp5 probes with nuclear extracts depleted of c-Jun or ATF2. Immunoglobulin G (IgG) was used as an antibody control. SB, specific DNA-protein band.