FIG. 2.

The deletion of HTZ1 leads to delayed peroxisome biogenesis. (A) The deletion of HTZ1 impairs cell growth on oleate-containing medium. Strains were grown to mid-logarithmic phase in liquid YPD medium, and equal amounts of cells were serially diluted 10-fold onto YPD and incubated at 30°C for 3 days and onto oleate-containing YPBO and incubated at 30°C for 5 days. (B) The fluorescent images of WT and htz1Δ cells shown are expressing the peroxisomal matrix protein Pot1p fused with GFP (Pot1p-GFP) at different time points of oleate incubation and were captured on a TCS SP2 laser scanning spectral confocal microscope.