FIG. 3.

Htz1p dynamically dissociates from oleate-responsive promoters upon induction. (A) POT1, POX1, FOX2, and CTA1 mRNA levels were determined by RT-PCR in WT and htz1Δ strains over a time course of oleate induction. The signal obtained from ACT1 mRNA was used as a loading control for normalization. Error bars represent standard deviations from the means of three independent experimental values. (B) Htz1p enrichment at four promoters was determined by qPCR during oleate induction. Relative enrichment values (y axes) are the averages of the results from three independent ChIPs with qPCR determination performed twice per biological replicate. Nonpromoter IGRi YMR325W was used as an internal control to normalize signals of promoter enrichment. In response to oleate induction, Htz1p was lost from the POT1, POX1, FOX2, and CTA1 promoters.