FIG. 5.

The acetylation of Htz1p is required for efficient transcriptional induction. (A) POT1, POX1, FOX2, and CTA1 mRNA levels were determined by RT-PCR in the WT, htz1Δ, and Htz1p K14A mutant strains over a time course of oleate induction. The signal obtained from ACT1 mRNA was used as a loading control for normalization. Error bars represent standard deviations from the means of three independent experimental values. (B) Enrichment of WT Htz1p and the Htz1p K14A mutant at four promoters was determined by qPCR during glucose and oleate induction for 6 h. Relative enrichment values (y axes) are the averages of the results from three independent ChIP experiments with two technical replicates of each. Nonpromoter IGRi YMR325W was used as an internal control to normalize signals of promoter enrichment.