FIG. 6.

Recruitment of TBP during oleate induction requires Htz1p. (A) The association of TBP with the POT1, POX1, FOX2, and CTA1 promoters was determined by ChIP using anti-Myc antibodies, followed by gene-specific PCR. The relative enrichment ratio is plotted at four time points (0, 1, 4, and 6 h) of induction in oleate. ACT1 was used as an internal control to normalize signals of promoter enrichment. Error bars show the standard deviation from three independent experimental values with two technical replicates of each. (B) Deletion of HTZ1 did not affect TBP expression during oleate induction. The WT strain and htz1Δ strains expressing genomically integrated TBP were grown in 2% glucose overnight and then transferred to oleate-containing SCIM medium at the indicated time points. Samples containing equal amounts of protein were analyzed by Western blotting with anti-Myc antibody to visualize TBP expression. A polyclonal antibody directed against Gsp1p was used as a loading control.