FIG. 7.

Htz1p regulates the occupancy of specific nucleosomes on the POT1, POX1, FOX2, and CTA1 promoters. The NuSA was used to determine the nucleosome positioning and density at the POT1, POX1, FOX2, and CTA1 promoters during oleate induction (the time of induction is indicated on the left) in the WT and HTZ1 deletion strains. Each point represents the relative protection of each PCR amplicon, quantified by real-time PCR and normalized to a centromeric control. The position of each amplicon (referenced to the middle of each amplicon) within the promoter is shown on the x axis. The approximate location of a nucleosome is represented by a gray circle with the nucleosome number referred to in the text shown above the circle.