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. 2009 Feb 23;29(9):2398–2408. doi: 10.1128/MCB.01737-08

FIG. 2.

FIG. 2.

CHIP siRNA upregulates the expression of myocardin-induced SM contractile genes. (A) SMCs were transfected with control or CHIP siRNA and cultured in 2% horse serum for 48 h. CHIP and myocardin mRNA and protein levels were analyzed by RT-PCR and immunoblotting as indicated. (B) SMCs were transfected and cultured as in panel A. The transcripts of SMC genes, including those for SM22α, SM α-actin, SM-MHC, and myocardin, were examined by RT-PCR. (C) Quantitative analysis of the mRNA transcripts in panel B (n = 3, means ± SEM). *, P < 0.05 versus control siRNA. (D) SMCs were transfected with SM22α reporter, control siRNA, or CHIP siRNA for 48 h. Luciferase (luc) activity was measured (n = 3, means ± SEM). *, P < 0.01 versus control siRNA. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.