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. 2009 Feb 23;29(9):2398–2408. doi: 10.1128/MCB.01737-08

FIG. 4.

FIG. 4.

Mapping of the interactive domain of CHIP and myocardin. (A) The domain of CHIP involved in binding to myocardin. GST fusion proteins were analyzed by immunoblotting with anti-GST antibody (top). The ability of the truncated CHIP fusion proteins to bind to Flag-myocardin expressed in 293 cells was analyzed by immunoblotting with anti-Flag antibody (middle). Ten percent of the input is shown in the bottom panel. (B) Schematic representation of the CHIP mutant proteins used in panel A. (C) The domain of myocardin involved in binding to CHIP. GST-CHIP fusion proteins were analyzed by immunoblotting with GST antibody (top). The ability of the truncated myocardin proteins expressed in 293 cells to bind to GST-CHIP was analyzed by immunoblotting with anti-Flag antibody (middle). Ten percent of the input is shown in the bottom panel. (D) Schematic representation of deletion constructs of myocardin used in panel C. TPR, tetratricopeptide repeat; Q, a stretch of glutamine residues; SAP, SAF-A/B-acinus-PIAS domain; LZ, leucine zipper.