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. 2009 Feb 6;191(8):2656–2667. doi: 10.1128/JB.01689-08

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Copyright © 2009, American Society for Microbiology

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FIG. 2. — Phenotypic analyses of C. jejuni wild-type and flgS(H141A) mutant strains. (A) Motility phenotypes of C. jejuni strains producing wild-type or mutant FlgS proteins in MH semisolid agar 24 h after inoculation. The strains used included wild-type strain 81-176 Sm^r (DRH212) (WT), 81-176 Sm^r ΔflgS (DRH460), and 81-176 Sm^r flgS(H141A) (DRH1323). (B) Arylsulfatase assays for analysis of expression of flaB::astA and flgDE2::nemo in C. jejuni 81-176 derivatives producing wild-type and FlgS mutant proteins. The results are the results of a typical assay in which each strain was tested in triplicate. The values reported for each strain are the average arylsulfatase activity ± standard deviation relative to the level of expression of each transcriptional fusion in 81-176 Sm^r ΔastA ΔflgS, which was defined as 1 arylsulfatase unit. For expression of flaB::astA, the strains used included wild-type strain DRH665 (81-176 Sm^r ΔastA flaB::astA) (WT), DRH939 (81-176 Sm^r ΔastA ΔflgS flaB::astA), and SNJ958 [81-176 Sm^r ΔastA flgS(H141A) flaB::astA]. For expression of flgDE2::nemo, the strains used included wild-type strain DRH533 (81-176 Sm^r ΔastA flgDE2::nemo) (WT), DRH936 (81-176 Sm^r ΔastA ΔflgS flgDE2::nemo), and SNJ956 [81-176 Sm^r ΔastA flgS(H141A) flgDE2::nemo]. The FlgS proteins produced by the strains are indicated below the graph.