TABLE 1.
Comparison of parameters for L. japonicus plants inoculated with M. loti strains R7A, R7A CAA1::pFUS2, and R7A CAA1::pFUS2-pFAJ1709/CAa
| Days p.i. | M. loti strain | No. of nodules plant−1 | Wt per nodule (mg)
|
Top-plant wt (mg)
|
Nitrogenase activity (μmol ethylene h−1 mg nodule fresh wt−1) | ||
|---|---|---|---|---|---|---|---|
| Fresh | Dry | Fresh | Dry | ||||
| 14 | R7A | 1.33 ± 0.10A | 0.06 ± 0.02A | 0.05 ± 0.01A | 10.11 ± 0.61A | 1.28 ± 0.01A | 4.79 ± 0.91A |
| R7A CAA1::pFUS2 | 1.61 ± 0.10A | 0.25 ± 0.07B | 0.08 ± 0.01A | 12.76 ± 1.86A | 1.48 ± 0.32A | 7.59 ± 0.85B | |
| 28 | R7A | 2.94 ± 0.34A | 0.65 ± 0.04A | 0.10 ± 0.01A | 29.72 ± 2.50A | 3.44 ± 0.35A | 6.54 ± 0.91A |
| R7A CAA1::pFUS2 | 4.37 ± 0.31B | 1.07 ± 0.13B | 0.14 ± 0.02A | 30.17 ± 2.21A | 4.06 ± 0.40A | 11.18 ± 1.49B | |
| R7A CAA1::pFUS2-pFAJ1709/CA | 3.01 ± 0.11A | 0.61 ± 0.08A | 0.11 ± 0.02A | 27.29 ± 1.27A | 3.31 ± 0.08A | 7.48 ± 1.10A | |
a
Measurements were done at 14 and 28 days p.i. The values are means of four and six replications (three plants each) ± standard errors (SE) for 14 and 28 days p.i., respectively. For the numbers of nodules, the values are means of 40 plants ± SE. The statistical analysis refers to comparisons between the two strains only within the same day. Different letters indicate statistical differences at a 95% level of significance.