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The rpoS gene with a minimal −24/−12 σ54-type promoter is sufficient to restore infectivity of the rpoS mutant in micea
| Strain or phenotype | No. infected/no. inoculated with indicated no. of bacteria
|
ID50 | |||||
|---|---|---|---|---|---|---|---|
| 101 | 102 | 103 | 104 | 105 | 106 | ||
| Wild type | 0/6 | 1/8 | 8/8 | 8/8 | ND | ND | 239 |
| RpoS− | ND | ND | ND | 0/8 | 0/8 | 0/8 | >106 |
| RpoS−/pJSB296 | 1/6 | 4/8 | 8/8 | 8/8 | ND | ND | 87 |
The ID50 values were calculated using the method described by Reed and Muench (16). ND, not determined.
