FIG. 2.

DNA-affinity purification of protein that binds to the fmgBC promoter region. (A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of protein purified from the AS fraction using fmgBC DNA (positions −104 to −29). The arrow indicates the major species in the APP after staining with silver. The numbers indicate the migration positions of molecular mass (in kilodaltons) standards. (B) EMSAs with ³²P-labeled fmgBC DNA (12 nM) spanning from positions −104 to −29 and proteins in the AS fraction or the APP. Arrowheads indicate the shifted complexes produced with the wild-type (WT) DNA fragment. No complex was observed with a DNA fragment bearing the ACCA to CAAC mutation at positions −67 to −64 (mutant).