TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Source or reference
Strains
E. coli
BL21(DE3)	F−ompT hsdSB(rB− mB−) gal dcm with DE3, a λ prophage carrying the T7 RNA polymerase gene	Novagen
SMhisMrpC2	BL21(DE3) containing pET16b/His10-MrpC2	35
SMFruAhis	BL21(DE3) containing pET11km/FruA-His6	35
EDYFruA	BL21(DE3) containing pET11a/FDBD-H8	68
M. xanthus
DK1622	Wild type	17
DK4499	Tn5 lac Ω4499	25
MSM1727.DZF1	sglA1 attB::pREG1727	This study
MDY1727.FA	sglA1 fruA::TnV Ω786 attB::pREG1727	68
MSM4499.DZF1	sglA1 attB::pDY52	This study
MSM4499.FA	sglA1 fruA::TnV Ω786 attB::pDY52	This study
Plasmids
pET11a/FDBD-H8	pET11a with a gene encoding FruA-DBD-His8 under the control of a T7 RNA polymerase promoter	57
pET16b/His10-MrpC2	pET16b with a gene encoding His10-MrpC2 under the control of a T7 RNA polymerase promoter	39
pET11km/FruA-His6	pET11km with a gene encoding FruA-His6 under the control of a T7 RNA polymerase promoter	S. Inouye
pREG1727	Apr Kmr P1-inc attP 'lacZ	7
pDY52	pREG1727 with 150-bp XhoI-BamHI fragment from pDY51	67
pDY100	pGEM7Zf with fmgBC DNA from positions −218 to +50	67
pDY133	pDY100 with a GCCGC-to-TAATA mutation at positions −81 to −77	67
pDY129	pDY100 with a GGAC-to-TTCA mutation at positions −71 to −68	67
pDY127	pDY100 with a ACCA-to-CAAC mutation at positions −67 to −64	67
pDY125	pDY100 with a CCGG-to-AATT mutation at positions −63 to −60	67
pDY49	pDY100 with a TCATTC-to-GACGGA mutation at positions −59 to −54	67
pDY121	pDY100 with a CCTTC-to-AAGGA mutation at positions −53 to −49	67
pDY47	pDY100 with a GAAC-to-TCCA mutation at positions −48 to −45	67
pDY117	pDY100 with a C-to-A mutation at position −37	67
pDY45	pDY100 with a CATTCCT-to-ACGGAAG mutation at positions −36 to 30	67