TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| CF1648 | Wild-type K-12 strain (MG1655) | 59 |
| CF1652 | ΔrelA251::kan derivative of CR1648 | 59 |
| CF1693 | ΔspoT207::cat derivative of CF1652 | 59 |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15ΔlacX74 recA1 araD139 galU galK Δ(ara-leu)7697 rpsL (Strr) endA1 nupG | Invitrogen |
| EC100D pir+ | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ−rpsL nupG pir+ (DHFR) | Epicentre |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] | 62 |
| Sphingomonas sp. | ||
| Rr 2-17 | Wild-type isolate from nopaline grapevine tumor | This work |
| Hx 699 | Mutant isolate 699, rsh::EZ-Tn5, Kmr derivative of Rr2-17, deficient in AHL synthesis | This work |
| A. tumefaciens NTL4(pZLR4) | Indicator strain for detection of AHLs | 7 |
| Chromobacterium violaceum | ||
| Wild type | ATTC 31532, produces violacein pigment and C6-HL signal | ATCC |
| CV026 | Indicator strain for detection of alkanoyl-AHLs, derivative of 31532 with mini-Tn5, Kmr, cviI | 31 |
| Plasmids | ||
| pHG1 | 12-kb EcoRI fragment from digestion of Hx 699 genomic DNA containing EZ-Tn5 transposon, Kmr | This work |
| pCR2.1 | pUC ori, Kmr Ampr | Invitrogen |
| pHG2 | Same as pCR2.1 but with EcoRI deletion | This work |
| pHG3 | pCR2.1 with rsh and native promoter, Kmr Ampr | This work |
| pRK290 | Broad host range, oriT and oriV, Tcr | 14 |
| pHG4 | pRK290 with rsh and native promoter, Tcr | This work |
| pSB401 | luxR+ PluxI-luxCDABE Tcr p15A ori | 58 |