FIG. 2.

Map of the thermosensitive replication plasmids carrying the P6 constitutive promoter and the various fragments of the ICESt1 recombination module. The location of the thermosensitive replication origin [Ori(pWV01^TS)] is indicated by a white rectangle. The location and the orientation of the ermB gene, encoding resistance to erythromycin, is indicated by a gray arrow. The PstI and KpnI restriction sites used for molecular cloning are indicated. The location and the orientation of the P6 constitutive promoter from Lactobacillus acidophilus ATCC 4356 are indicated by a right-angle arrow. Black arrows indicate the locations and orientations of the int and xis genes, encoding the integrase and the excisionase, respectively. The location and the orientation of a fragment corresponding to the last 60 nucleotides of the fda gene is indicated by a black triangle. Recombination sites are drawn as rectangles: black, sequence identical in attL, attR, and attI sites; checkerboards, arm of attL sites and related arm of attI sites; hatched boxes, arm of attR sites and related arm of attI sites. The recombination sites are magnified. The putative rho-independent terminator downstream from the int gene is indicated by a lollipop. The locations and orientations of the primers AttI2 and AttI3 are indicated by white arrowheads (see Table S1 in the supplemental material).