FIG. 3.

Characterization by PCR of an intraspecies ICESt3 transconjugant. CNRZ385 ICESt3cat and LMG18311/pMG36e were used as the donor and the recipient, respectively. Don, Rec, and Tc indicate donor, recipient, and transconjugant, respectively. (A) ICESt3, amplification of a fragment of the regulation module. cse, amplification of the central part of the cse gene. (B and C) attR, attL, attB, and attI, amplifications of fragments carrying these attachment sites. The sizes of the PCR fragments are deduced from sequence analysis and confirmed by comigration with a DNA ladder. The classes of primer pairs used for these amplifications are indicated in parentheses, and their localizations are indicated in Fig. 1.