FIG. 6.

Schematic representation of the site-specific integration of plasmid pNST260+ into the fda 3′ end and characterization of an E. faecalis transformant carrying this plasmid. (A) The locations and orientations of primers hybridizing to the chromosome and to the plasmid are indicated by black and white arrowheads, respectively. The recombination sites are magnified. The primer pairs A-AttI3, AttI2-F, A-F, and AttI3-AttI2 allow the amplification of fragments carrying the attL, the attR, the attB, and the attI sites, respectively (see Table S1 in the supplemental material). The classes of primer pairs used for these amplifications are indicated. The symbols used to represent plasmid pNST260+ are identical to those used in Fig. 3. (B) Amplifications by PCR using the DNA of E. faecalis JH2-2 and a clone of E. faecalis JH2-2 carrying plasmid pNST260+. attRç attL, and attB, amplifications of fragments carrying these attachment sites.