FIG. 1.

(A to C) Arrangement and expression of sle genes in C. perfringens SM101. (A) The arrangement of sleC and sleM in C. perfringens SM101 is shown, and the locations of the primers used to amplify the upstream regions of each gene are indicated. The sleC and sleM promoters were predicted to be within the intergenic regions between sleC and cspB and between sleM and CPR1312, respectively (18). (B and C) GUS specific activities from sleC-gusA (B) and sleM-gusA (C) fusions in C. perfringens SM101 grown in TGY vegetative (▵) and DS sporulation (▴) media were determined as described in Materials and Methods. The data represent averages of three independent experiments, and time zero denotes the time of inoculation of cells into either TGY or DS medium.