TABLE 1.
Colony formation by spores of C. perfringens strainsa
| Strain/genotype | Spore titer (CFU/ml/OD600) onb:
|
|
|---|---|---|
| BHI | BHI + Lyzc | |
| SM101/wild-type | 4.0 × 107 | 8.9 × 107 |
| DPS107/sleC | 1.4 × 104 | 3.9 × 107 |
| DPS107(pDP138)/sleC carrying wild-type sleC | 2.7 × 107 | ND |
| DPS109/sleM | 3.4 × 107 | ND |
| DPS110/sleM sleC | 1.6 × 102 | 6.6 × 107 |
a
Heat-activated spores of various strains were plated on BHI agar, and colonies were counted after incubation at 37°C for 24 h.
b
Titers are the average of CFU/ml/OD600 determined in three experiments, and the variance was <25%.
c
Spores were decoated and plated on BHI plates containing 1 μg of lysozyme (Lyz)/ml. ND, not determined.