TABLE 3.

Oligonucleotide primers used in this study

Primer	Restriction site	Sequence (5′-3′)a	Application
AP3	BamHI	ATATAGGATCCCGTGGACCTGTCCCTG	Forward primer for creation of bldG-containing pKT25-, pUT18C-, and pUT18-based constructs
AP4	KpnI	ATATAGGTACCCGTCAGTCGGTGGCCGC	Reverse primer for creation of bldG-containing pKT25- and pUT18C-based constructs
AP11	KpnI	ATATAGGTACCCGGTCGGTGGCCGCCAC	Reverse primer for creation of bldG-containing pUT18-based constructs
AP12	BamHI	ATATAGGATCCCATGGCCACCGTCGAACTCCGT	Forward primer for creation of sco3548-containing pKT25-, pUT18C-, and pUT18-based constructs
AP13	KpnI	ATATAGGTACCCGTCAGATCGGTGGCAGCACCGC	Reverse primer for creation of sco3548-containing pKT25- and pUT18C-based constructs
AP14	KpnI	ATATAGGTACCCGGATCGGTGGCAGCACCGCCGA	Reverse primer for creation of sco3548-containing pUT18-based constructs
AP7		GGCGCGCAGTTCGGTGACCAGCGGC	Forward primer for sequencing pKT25 inserts
AP8		GGGATGTGCTGCAAGGCGATTAAG	Reverse primer for sequencing pKT25 inserts
AP9		TTTATGCTTCCGGCTCGTATGTT	Forward primer for sequencing pUT18 inserts
AP10		CAAGTCGATGCGTTCGCGAT	Reverse primer for sequencing pUT18 inserts
AP30		CTCGCCGGATGTACTGGAAAC	Forward primer for sequencing pUT18C inserts
AP31		CGGGGCTGGCTTAACTATGC	Reverse primer for sequencing pUT18C inserts
BKL62	EcoRI	CGGCGAATTCGTCTGCAACCAGGAGCGC	Forward primer for pAU365 construction
DBG3	XbaI	GCGCTCTAGAGTTCGACGGTGGCCATG	Reverse primer for pAU316 construction
DBG33	XbaI	GCGCTCTAGATCCCATGCCATTGATCGTGAA	Reverse primer for pAU317 construction
DBG34	NdeI	GCGCCATATGGCCACCGTCGAACTCCGT	Forward primer for pAU317 construction
DBG35	NdeI	GCGCCATATGGACCTGTCCCTGTCGAC	Forward primer for pAU316 construction
KC61	XbaI	CGCGAGATCTGTCCGCACGGCACCAATG	Reverse primer for pAU365 construction

^aRestriction enzyme sites are underlined.