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. 2009 Feb 13;191(8):2691–2702. doi: 10.1128/JB.01182-08

FIG. 3.

FIG. 3.

Degradation of a 5′-labeled or 3′-labeled 20-nt synthetic RNA by the HP1248 protein. (A) Degradation of 3′-labeled unmodified RNA. For the times indicated, 0.5 pmol RNA labeled at the 3′ end was incubated with 0.1 mg His-tagged HP1248. A control reaction (M) was performed with the HP1248 protein D231N mutant for 30 min. (B) Degradation of 5′-labeled unmodified RNA. For the times indicated, 0.5 pmol RNA labeled at the 5′ end was incubated with 0.1 mg His-tagged HP1248. A control reaction (M) was performed with the HP1248 protein D231N mutant for 30 min.