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. 2009 Feb 13;191(8):2691–2702. doi: 10.1128/JB.01182-08

FIG. 5.

FIG. 5.

HP1192 is required for H. pylori motility. (A) Photograph of a Brucella broth-10% FBS soft agar plate after 4 days of incubation. WT, HP1248−, HP1192−, and HP1248− HP1192− indicate the wild type, C1/HP1248-cat mutant strain, C1/HP1192-km deletion mutant strain, and HP1248/HP1192 double mutant, respectively. HP1192++ and flaB++ indicate the HP1192 overexpression strain and flaB overexpression strain, respectively. (B) Slot blot. The amount of RNA was standardized at 10 μg per slot. RNA extracted from the H. pylori WT (lane 1), HP1248 deletion mutant (lane 2), HP1248 complementation strain (lane 3), HP1192 overexpression strain (lane 4), flaB overexpression strain (lane 5), HP1192 deletion mutant (lane 6), or HP1248− HP1192− double mutant (lane 7) was assayed in the respective experiments performed with probe specific for HP1192 genes. RNA slot blot analysis with labeled probes specific for ureA was performed as an internal control. (C) Quantitative analysis of triplicate HP1192 RNA expression normalized with ureA RNA expression.