FIG. 1.

Analysis of Igl protein synthesis by F. tularensis strains. Igl proteins in the pellet fraction were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by immunoblotting using antiserum specific for IglA, IglB, IglC, or IglD. Lane a, wild-type strain LVS; lane b, ΔiglA null mutant; lane c, complemented ΔiglA mutant (pJEB415); lane d, ΔiglB null mutant; lane e, complemented ΔiglB mutant (pJEB416). The asterisk indicates a protein band that cross-reacts with the anti-IglD antiserum. The experiment was repeated at least three times, and the results of a representative experiment are shown. α-IglA, anti-IglA; α-IglB, anti-IglB; α-IglC, anti-IglC; α-IglD, anti-IglD.