FIG. 4.

Analysis of Igl protein synthesis for an iglA null mutant expressing wild-type or mutated IglA in trans. Igl proteins in the pellet fraction were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by immunoblotting using antiserum specific for IglA, IglB, or IglC. (A) Expression profiles for IglA deletions mutants, divided into three phenotypic groups (strong, poor, or abolished) based on their ability to interact with IglB in yeast. (B) Expression profiles for alanine substitution mutants. Mutants with more pronounced defects in IglB binding are indicated by an asterisk. The experiment was repeated at least two times, and the results of a representative experiment are shown. α-IglA, anti-IglA; α-IglB, anti-IglB; α-IglC, anti-IglC.