FIG. 5.

Intracellular growth of strains of F. tularensis. J774 cells were infected with various strains of F. tularensis at a multiplicity of infection of 200 for 2 h. After gentamicin treatment, cells were allowed to recover for 30 min, after which they were lysed immediately (corresponding to 0 h) (gray bars) or after 24 h (black bars) with a phosphate-buffered saline-buffered 0.1% sodium deoxycholate solution and plated to determine the number of viable bacteria (log₁₀). All infections were repeated three times with triplicate data sets. The results of a representative experiment are shown. The bars indicate the means, and the error bars indicate the standard deviations.