FIG. 2.

Analysis of motility, polar flagellin, and LPS for A. caviae Sch3N and isogenic mutants. (A) Motility as assessed by determining a strain's ability to swim in 0.3% semisolid motility agar. WT, wild type. (B) (Upper panel) Polar flagellin immunoblot of whole-cell proteins of A. caviae Sch3N (WT) and flm locus isogenic mutants. (Lower panel) Polar flagellin immunoblot of whole-cell proteins of flm locus isogenic mutants complemented with individual copies of the wild-type genes in pBBR1MCS (Table 1). The genes in which the knockout occurs are indicated above the lanes. Proteins were obtained from bacteria grown at 37°C in BHIB and were analyzed by SDS-PAGE (12%). They were transferred onto nitrocellulose membranes and immunoblotted with anti-polar flagellin antibodies (1:500). (C) Analysis of LPS isolated from A. caviae Sch3N (WT) and the flm locus isogenic mutants. (Upper panel) The genes in which the knockout occurs are indicated above the lanes. (Lower panel) flm locus isogenic mutants complemented with individual copies of the wild-type genes in pBBR1MCS (Table 1). LPS was extracted from bacteria grown at 37°C in BHIB, analyzed by SDS-PAGE (12%), and silver stained. The positions of LPS bands A and B are indicated on the left.