Skip to main content
. 2009 Feb 20;191(8):2485–2492. doi: 10.1128/JB.01734-08

FIG. 2.

FIG. 2.

Effect of glutamine on the in vitro ability of wild-type and mutant GS proteins to alter DNA binding by GlnR and TnrA. (A) The activation of GlnR binding to glnRA promoter DNA was determined with a gel mobility shift assay. The GlnR and GS promoter concentrations were 25 nM and 20 μM, respectively. (B) The inhibition of TnrA binding to tnrA promoter DNA was determined with a gel mobility shift assay. The TnrA dimer and GS subunit concentrations were 100 nM and 1 μM, respectively. The symbols for the different GS proteins are as follows: wild type, ○; V173I, □; L174F, ▵; and V190A, ⋄. Each data point is the mean of at least two independent experiments and is reproducible to ±10%.