FIG. 4.
Growth curves of WP3 strains. (A and B) Growth characteristics of the EPA-deficient strain S. piezotolerans WP3ΔEPA at low temperature (4°C) (A) and high pressure (20 MPa) (B) in the absence or presence of exogenous EPA at a final concentration of 0.025%. (C and D) Effects of cerulenin (10 μg/ml) on the growth of S. piezotolerans WP3 at various temperatures (C) and pressures (D). (E and F) Growth of the mutant strain S. piezotolerans WP3ΔLIV at various temperatures (E) and pressures (F). OD600, optical density at 600 nm. The error bars indicate standard deviations.